Health, husbandry, and captive breeding of Golden Greek Tortoises

David S. Lee and Mike Lowe, © 2007 The Tortoise Reserve

 

Introduction:

 This note is based on a captive group of Golden Greek Tortoises we
 purchased from reptile dealers between April and August 2002 and an
 individual donated from a tortoise rescue group in Maryland. The
 animals were acquired through three different sources. The composite
 group consisted of 6.6 adult tortoises. The males range from 360-430
 grams and 126-130 mm standard carapace length. The females range from
 800-1045 grams and 155-165 mm standard carapace length. Taxonomic
 arrangement of the Greek Tortoise complex is confusing and unsettled. At
 this time Golden Greek Tortoises (a popular hobbyist trade name) are
 recognized as Middle Eastern spur-thighed  tortoises, Testudo (graeca) terrestris   
 Forskal, 1775. The founder stock of our group is believed to come from Syria, but they
 also occur in Turkey and northern Iraq.

 According to Bonin, et al. (2006 Turtles of the World) no studies have
 been published on this tortoise.

 Medical treatment:

 Immediately upon acquisition all tortoises were dewormed with panacure.
 Dosage was 50mg/kg for three consecutive days. This same dosage was
 repeated two weeks later. Each was also treated with enrofloxacin for
 respiratory ailments, a problem with all imports we examined. Dosage was
 5mg/kg daily over a course of 3 weeks. Three of our tortoises continued
 to exhibit occasional reoccurring runny noses. This condition seemed to
 coincide with dampness, high humidity and foods with high moisture
 content. Individuals were retreated with enrofloxacin as needed. A group
 maintained outside throughout the year also occasionally showed signs of
 reoccurring respiratory problems but they were not retreated. Their
 respiratory problems seemed to be resolved through their own
 thermogulatory behavior. Two tortoises in the outdoor group died, but
 based on the timing of death neither mortality seemed to be associated
 with hibernation. A female died of unknown problems, and one male died
 which on examination showed extensive liver liaisons. This male was
 active and feeding the week prior to its death. Both these individuals lived
 approximately one year under our care. At the time of this writing
 the remaining tortoises in our founder group all appear to be healthy.
 Neither group has been in contact with any other species of chelonian
 since acquisition.

 

Housing:

 Our tortoises are divided into two groups, each is managed differently.
 One group is left outside throughout the year, the other is moved from
 out door to in door enclosures based on season and weather. Thus far we
 have confirmed reproduction in both group, but we have not had successful
 hatchling of eggs left in the ground, and while we have certainly missed
 finding some clutches no hatchlings have appeared in the pens. Apparently
 local weather conditions or soil types fail to provide adequate or consistent
 incubation conditions. Hatchlings and young are kept inside during winter
 months.

 One group is kept outside primarily during the summer. They are brought
 in during extended rainy periods, and at night in the spring and fall
 when the night temperatures are expected to drop below 65 F or when
 there is heavy dew. The winter, in door enclosure is 3 x 5 feet, the
 outside enclosure is 4 by 6 feet (six adult tortoises). The outside
 enclosure is a box frame made from pressure treated 2 x 10 boards and
 covered with a wire top. A 12-inch board is mounted across one end for
 shade. The frame has no bottom and is daily moved about a mowed lawn,
 providing constant availability of new vegetation growth for the tortoises.
 The inside enclosure is illuminated by a 60 watt bulb for 11-12 hours a day,
 and heated by a 100 watt ceramic heater which stays on constantly. Even
 during the winter the tortoises are put out doors 1-2 times a week on
 warm sunny days.

 The second group lives outside throughout the year. The weather in SE
 North Carolina is mild in the winter and hot and humid in the summer. It
 regularly gets below freezing from December through February. Frost
 typically begins in November. We should note that 2002 was the driest
 year on record for this part of the state and 2003 was the wettest. A
 rain gauge is mounted on the pen’s fence and total rain is recorded
 weekly. (On a week in mid April and again in early August we had 4.5
 inches of rain, and many weeks we had 2 or more inches) No signs
 of poor health were associated with any extreme weather conditions. The
 tortoises are housed in a pen with southern exposure, the total area is
 just over 300 square feet, with two separate sheltered areas under boxed
 in steps and under a small elevated building. The building is closed in
 at ground level and the areas accessible to the tortoises under the
 building and steps are each approximately 2.5 x 3 feet. The protected
 area under the steps is open on the south side for the tortoises to have
 access, the one under the house has only a small 8 x 8 inch opening for
 access. Both of the protected areas have about 3 inches of cypress mulch
 over a dirt floor. The pens’ fencing is about 2.5 feet high and is
 buried into the ground about 10 inches. It is made of pressure treated
 wood. The fence is built as a solid visual barrier so the tortoises do
 no constantly walk the pen’s perimeter. Above the fencing at 1 inch, 1
 foot, and 2 foot intervals are strands of 20 gauge wire for an electric
 fence. A ground wire was installed several inches above the soil on the
 outside of the pen. Despite the presence of bobcats, raccoons, gray
 foxes, coyotes, feral dogs, and black bears on the property we have had no
 problems with predation. Several dirt mounds, tree trunks and shrubs are
 present in the pen and portions of the pen are shaded at various times
 throughout the day by several pine trees growing in and adjacent to the
 pen. The tortoises use the mounds and the fencing to angle themselves
 for maximum exposure to solar radiation early in the morning. The soil
 is a loose sandy loam, which drains quickly. Even during periods of
 heavy rain no standing water has been observed. Several inches down the
 soil is sometimes quite moist during, but the drainage is probably a
 key factor in keeping the tortoises healthy during wet periods. Despite
 frequent seeding, by the end of the summer the ground is 85% bare except
 for some low growing evergreen Smilax bona-nox, the tortoises don’t eat
 this but it provides good cover. Other native and naturalized plants
 growing in the pen, which are not eaten, are probably two tall for the
 tortoises to reach. Like other Testudo they are grazers and not
 browsers. These tortoises are seldom handled, and when active will run
 for cover when they see people approach. They spend long periods sitting
 in the same relative positions, and each tortoise has three or four
 preferred stations where they spend a good portion of each day. In an
 adjacent and similarly constructed pen which faces SE and abuts the same
 building a captive group of adult Testudo (graeca) lbera are maintained.
 Their behavior, periods of activity, and thermal regulation are similar
 through they are less active on cooler days than the Golden Greeks.
 These tortoises are also kept outside throughout the year. No artificial
 heating devices are used on either group.

 

Diet:

 Considering their small size these tortoises eat large amounts of food
 daily. None were observed to drink from open water containers nor did
 they show any interest in soaking. We have been unable to get either
 group of these tortoises to feed on commercial tortoise chows, though
 the same brands are eaten by other taxa of T. gracea in our collection.
 Fruits are seldom offered, and not eaten with any frequency when they
 are. We have not seen them eating mushrooms. When maintained outside
 these tortoises feed mostly on plants growing in their enclosures with
 supplemental feedings of grocery store produce at least once a week (see
 list below). The tortoises typically walk about eating small amounts of
 native and naturalized vegetation and then move a foot or two before
 they continue foraging. New broad-leafed vegetation planted in the pen
 is soon discovered and usually completely consumed that day. The
 differences in vegetation, both in density and in diversity is striking
 when comparing what is growing naturally outside vs inside the fence.
 The other group is housed outside in a movable pen so that fresh plant
 growth is always available. ‘Naturally growing’ food items include
 clover, dandelions, plantain, and various grasses. This is supplemented
 with squash, hibiscus flowers, carrots, corn, okra, and dark green
 lettuce (for its moisture content). In the late fall when the tortoises
 are less active the pen in which tortoises are housed throughout the
 year is seeded with grasses, clover, and seed mixes used for wildlife
 food plots. Note that the less expensive lawn seed has a higher content
 of seeds of lawn type ‘weeds’ and this is there for preferred for tortoise
 pens when compared to high grade more expensive seed. The pen’s interior
 is lightly fertilized in early spring. Actually the care of the ground
 so that it supports the best possible forage crop possible is more work
 than the actual husbandry of the tortoises. In the winter the group that
 is not hibernated is fed primarily on endive, various dark lettuces,
 collards, and squash supplemented with calcium powder.

 When the tortoises are outside food is randomly scattered throughout the
 pen. When the tortoises are fed indoors in smaller containers the food is
 placed in a liner fashion so that all animals have access to the food
 simultaneously. This is in contrast to dish feeding where three or four
 tortoises have primary access to the food. Uneaten food is removed a few
 hours after feeding.

 When inside the tortoises are fed almost daily but this is not necessary, it is
 more an artifact of activity in the care of other species housed in our collection.
 Tortoises housed outside throughout the year are not fed from late October
 through mid to late March, though by March they are frequently seen grazing on
 various plants that are naturalized in their pens.

 

Behavior:

 Basking: Tortoises bask during mid morning hours, with exact times
 varying with the season and weather. During the heat of the day they
 seek shade or cover, during cool periods they move from spot to spot as
 patches of direct sun shift through out the day. On intermittent cool
 cloudy days they stay primarily inside their retreats. During cool
 weather in the fall the tortoises will dig into the substrate seeking
 sites and positioning themselves so that the morning sun warms the
 exposed posterior portion of their shells. By the time the air
 temperature is in the high 60s F, the ground temperature in areas where
 the pen is receiving full sun is often 85 F and by the time air
 temperature is in the low 70s the tortoises are in places where the
 temperature is 90 F. During cool periods the tortoises are most active
 from late morning through early afternoon, during the summer they
 exhibit a bipolar activity pattern and are inactive during the hottest
 parts of the day.

 Hibernation: By mid October the tortoises start excavating shallow
 burrows in spots that will be heated by the morning sun. They continue
 to feed but do not eat much compared to what they consume when ambient
 temperatures are consistently higher. Once daytime temperatures are
 consistently in the mid 60s F or lower the tortoises become inactive
 (typically the first week of November). They either take shelter under
 the building in the protected areas with a substrate of dry cypress
 mulch, or remain partially to completely burrowed outside. Often the
 tortoises will remain outside throughout the winter (particularly the
 females) and the tops of their carapace are exposed to the air during
 hibernation (in much the same fashion that many of our native box
 turtles hibernate). When removed from these sites and placed inside the
 more protected areas under the building within a few days they are back
 in their original hibernation site. We have learned to just leave them
 be assuming they are better aware of their thermal needs than we are. A
 long term captive group of T. (gracia) libera maintained in an adjacent
 pen exhibit similar winter behavior patterns. In 2003 the Golden Greeks
 emerged from hibernation on 8 March (the same date that the first
 Iberian Greek Tortoises and Herman’s tortoises were seen. The first
 Russian Tortoises emerged on 14 March that year. All are in separate
 pens but on are in the same general vicinity of our property). The
 Golden Greek and Iiberian Tortoises also make brief appearances on
 unseasonably warm days in November and on December while the Golden
 Greek tortoises during warm sunny days continue to emerge to bask for a few
 Hours throughout the winter.

 Group wintering indoors: Despite being moved in and out, and from pen to
 pen on a regular basis these turtles show no sign of stress and seem
 content with the routine, often feeding and mating within minutes of
 being moved. This is contrary to what some authors have suggested as
 they insist that the captive tortoises should be handled and moved as
 little as possible in order to assure consistency and to achieve maximum
 reproductive success.

 

Reproduction:

 Mating: If the animals in our group are typical adults this taxa is
 perhaps the most sexually dimorphic in size of any of the T. gracia
 complex. The largest females have a mass that is nearly 2.5 times larger
 than that of the largest males. The mating behavior of Golden Greek
 Tortoises seems no different from that of other taxa of T. gracea. Males
 follow and ram females prior to mounting. Mounted males vocalize but not
 as loudly as the larger taxa. Often they just ram other tortoises (males
 and females) as a determination of dominance and this frequently appears
 to have no direct relationship to reproduction. Mating is stimulated by
 moving the tortoises to new quarters, and increased temperatures. Mating
 occurs throughout the year in the group that is housed inside during the
 winter and from mid-April through late-October in those kept outside
 throughout the year. Ramming and associated activities occur mostly at
 ground temperatures above 80 F, frequently, and at any time during
 daylight hours. Male aggression seems to be limited and minor, but a
 number of visual barriers are present in the pens and this probably
 decreases male to male interactions.

 Nesting and eggs: Females ready to lay paced their enclosures and rammed
 other tortoises. Eggs were laid in lawns or when available sand boxes.
 Nests were excavated to three inches and the eggs were deposited in
 flask shaped holes. The entire nesting procedure lasted up to three
 hours. Most eggs were laid in late morning; data on 7 clutches from four
 females as follows. Clutch size 2-3 eggs (mean 2.28) Second clutches
 from same females laid about 30 days after first (n=2). Most eggs were
 produced in June and July although one fertile three egg clutch was laid
 in December. Eggs weighed 16.6-19.7 (mean 18.54) grams and measured 37.4
 - 42.8 x 27.6 - 29.5 (average 40.23 x 28.55) mm. One clutch was broken
 by the female while covering the eggs, a second clutch was broken by us
 digging them up. The first four clutches that hatched produced 9 young.
 Information on subsequent clutches (post 2003) was not recorded.

 Incubation: The first clutch of eggs produced (Dec. 2002) was incubated
 under conditions which have proven successful for hatching T. graeca
 gracea. These three eggs were fertile and split because of too much
 moisture in the vermiculite. Subsequent clutches were incubated on dry
 vermiculite at a temperature of 86 +/- 1 F. An air humidity of 60-80%
 was maintained in the incubator and eggs were lightly misted on days 40
 and 65. This combination of micro environmental manipulations resulted a
 100% hatchling success with tortoises emerging between 65-76 days. Young
 typically remained in the egg 24-48 hours. Unlike our captive T. g. Iberia,
 undetected clutches, and ones left in place in their enclosures failed to hatch.
 In that the Iberean Tortoise eggs hatched when left in place and the Golden
 Greek do not, we assume factors contusive to successful incubation differ
 between these two tortoises..

 Hatchlings: Newly hatched young, like the adults, are pale both in shell
 color and on their heads and appendages when compared to hatchlings of
 the nominate. Hatchlings weighed 13.9-19 (mean 16.3) grams. They
 measured 38.3-41.6 (SCL) x 33.2-36.0 (carapace width) mm. Yolk sacks
 were well absorbed at the time of emergence from the egg. All hatchlings
 started eating with in 24 hours. Several were eating with in hours of
 hatching. All indications are the young are healthy and fast growing. We
 keep them on moist cypress mulch, the high humidity results in better shell
 growth than when the young are maintained on drier substrates, The
 young produced from our first two seasons of breeding proved to be all
 males and we are now experimenting with incubations temperatures but
 the young tortoises produced subsequently are too still too young to sex.

 

Summary:

 This is apparently the first documented successful breeding of Golden
 Greek Tortoises in captivity. In that we see no major deviation from the
 documented husbandry and breeding of other temperate Testudo graeca
 taxa, compared to the vast number of wild caught adults of these turtles
 on the market during the late 1990s and first few years of this century,
 we assume that our success is based primarily in our initial deworming
 and aggressive control of respiratory ailments in the imported stocks.
 This conclusion is strengthened by the relatively high mortality rate of
 imports. Our two distinct and successful captive husbandry techniques
 suggest a wide latitude of conditions in which captive animals can be
 successfully maintained once stabilized. Breeding of the group maintained
 year round out of doors in a temperate setting deserves additional monitoring,
 but preliminary information suggest that this is a viable strategy for
 captive groups of Golden Greek Tortoises in appropriate climates and
 well drained soils.

 

Acknowledgement:

 We thank the late Dr. Barbara Bonner, who visited our facility in March
 2003 and made many useful observations which were of long term value to our 
 husbandry methods of these and other chelonians in our collection.

 

 

Published in World Chelonian Trust Newsletter No.7